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HPLC purity % with related-impurity peaks listed. Baseline COA — public + accession #.
Purity + LC-MS identity + Net Peptide Content + TFA residual. The research-grade receipt.
Plus USP <85> endotoxin, USP <71> sterility, heavy metals, residual solvents. The injectable-grade COA.
Two: (1) related-peptide impurities, especially the des-Gly variant from incomplete N-terminal coupling, which can run 2-5 % of total area and is missed by HPLC-only panels with weak gradient resolution; and (2) Net Peptide Content gap — research-grade BPC-157 averages 76-86 % NPC, so a vial labeled '5 mg' usually carries 3.8-4.3 mg of active. The Identity + Potency panel ($499) catches both because it includes LC-MS (for impurity speciation) and NPC.
Both are tested the same way. Identity by LC-MS is identical (same parent ion mass), and HPLC purity is identical. The salt form changes the counter-ion (acetate vs arginate vs TFA), so the TFA / acetate residual assay reports against whatever's actually in the vial. Arginate is less common; acetate is the most-frequently-encountered. Note both salt forms on the submission so we tune the IC method appropriately.
We can confirm intact peptide content in lyophilized form at receipt and on reconstitution; oral bioavailability and gastric stability are separate clinical pharmacology questions we don't address. For accelerated-stability work on lyophilized vials (storage at 25 °C / 40 °C / 60 % RH at intervals), we offer add-on stability time points for $99 per pull.
Purity ($199): single 2 mg sealed lyophilized vial. Identity + Potency ($499): same single vial. Full Disclosure ($899): two additional vials for the destructive sterility and endotoxin tests. Send the original sealed vials by overnight courier — lot and batch labels matter for chain of custody.
Janoshik publishes BPC-157 testing at around $300 for purity-only. Vanguard publishes $349. Our Purity panel is $199. Where the difference shows is at the Identity + Potency tier — our $499 includes LC-MS, NPC, and TFA together on one COA, where competitor pricing often splits these across separate orders. Everything we publish is on a public accession-verifiable COA at coa-lookup.
Yes. Combination products like 'BPC-157 + TB-500' or 'BPC-157 + TB-500 + GHK-Cu' (sometimes called 'GLOW') are tested with two-component or three-component HPLC quantification on a single chromatogram. Each component reported with its own purity %, retention time, and identity. Add one tier up from the Purity panel if you're submitting a blend — the multi-component method is included in Identity + Potency and higher.
Thymosin β4 fragment — the other half of the popular BPC + TB blend. Same HPLC + MS + NPC bench.
GLP-1 receptor agonist (Ozempic / Wegovy active). Same HPLC + MS + NPC + TFA bench.
Full peptide menu — all bundles, panels, and compounds across all peptide classes we cover.
HPLC purity, LC-MS identity, NPC, TFA, endotoxin, sterility — Houston bench, public COA, transparent pricing.