Semaglutide — what's actually in the vial?

HPLC purity tells you what fraction of the chromatogram peaks is the main Semaglutide peak vs related-impurity peaks. It's a relative measurement of peak area. Net Peptide Content tells you what fraction of the dry mass in the vial is actual active Semaglutide vs TFA / acetate counter-ions, water of hydration, and inorganic salts. A vial can have 99 % HPLC purity and only 78 % Net Peptide Content — both numbers matter for different reasons. Public COAs that only report purity are leaving out the dose-accuracy half of the story.

Yes. LC-MS identity confirmation runs on every Identity + Potency panel ($499) and above. We compare the observed molecular ion against the theoretical monoisotopic mass of 4113.58 Da and report mass accuracy in ppm. The full charge-state envelope is captured; any off-mass species (oxidations, methylations, sequence truncations) are flagged with their observed mass.

Industry-typical TFA / acetate counter-ion content runs 1-3 % w/w of the dry mass. Above 5 % usually indicates inadequate post-synthesis ion-exchange and causes both dose-accuracy distortion (active mass is over-estimated by TFA mass) and injection-site stinging. We report exact % w/w with the method documented on the COA.

Yes. Ship reconstituted vials cold-packed in the original BAC water or sterile saline. We can run NPC, LC-MS identity, and endotoxin on the reconstituted product, which is the most meaningful test for actual user exposure — but cold-chain and shipping volume make it less common than lyophilized testing. Pricing matches the dry vial panel.

Not legally required for research-use-only material, but the USP <71> sterility test is what separates injectable-grade material from research powder. Brands selling lyophilized peptide vials to consumers / clinics frequently include sterility because the regulatory expectation around injectables is what their buyers ask for. The 14-day membrane-filtration cultured-broth method is included in the Full Disclosure panel.

For Purity ($199): single 2 mg sealed lyophilized vial. For Identity + Potency ($499): the same single vial — we re-use the dissolved material across HPLC, LC-MS, NPC, and TFA. For Full Disclosure ($899): two additional vials (one for sterility, one for endotoxin, both destructive tests). Ship by overnight courier with original packaging — lot, batch, and seal integrity matter for chain of custody.